Manchester City were the first club to formally pull out after Chelsea had signalled their intent to do so by preparing documentation to withdraw.
The other four sides – Arsenal, Liverpool, Manchester United and Tottenham – have all now followed suit
The 12-team Super League was announced on Sunday to widespread condemnation.
Manchester City confirmed they have “formally enacted the procedures to withdraw” from the Super League.
Liverpool said their involvement in the proposed breakaway league “has been discontinued”.
Manchester United said they had “listened carefully to the reaction from our fans, the UK government and other key stakeholders” in making their decision to not take part.
Arsenal apologised and said they had “made a mistake”, adding they were withdrawing after listening to their fans and the “wider football community”.
Tottenham chairman Daniel Levy said the club regretted the “anxiety and upset” caused by the proposal.
All Six Premier League Teams Withdraw From ESL Competition